evaluation of cytokine mrna expression in vaccinated guinea pigs with foot-and-mouth disease type o inactivated vaccine
نویسندگان
چکیده
foot-and-mouth disease (fmd) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. this study quantifies the cytokines mrna expression of vaccinated guinea pigs withfmd type o inactivated vaccine. blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. extracted mrnas were reverse-transcribed into cdna and analyzed for quantification of ifn-γ, tnf-α and il-10 expression using relative real-time pcr assay. our results showed that all of the genes wereupregulated. the expression of tnf-α and il-10 genes significantly increased (p
منابع مشابه
Evaluation of cytokine mRNA expression in vaccinated guinea pigs with foot-and-mouth disease type O inactivated vaccine
Foot-and-mouth disease (FMD) is a severely contagious viral disease that mainly affects cloven-hoofedlivestock and wildlife. This study quantifies the cytokines mRNA expression of vaccinated guinea pigs withFMD type O inactivated vaccine. Blood samples were collected from eight guinea pigs at 7 and 28 days after thefirst vaccination. Extracted mRNAs were reverse-transcribed into cDNA and analyz...
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archives of razi instituteجلد ۷۱، شماره ۱، صفحات ۱۵-۱۹
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